Download or read book Identifying Genes Required for the Formation of Neurons from Skin Cells Using Forward Genetic Screens and Whole Genome Sequencing in C. Elegans written by Gregory Minevich and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The human brain is the most complex structure in the known universe and one of the ultimate goals of humanity is to understand its function. The "bottom-up" approach to developmental neuroscience seeks to assemble a "parts list" of the genes expressed in each neuron and a map of the gene regulatory networks that determine the identity of the diverse neuronal types. A key part of building such a gene regulatory map is to identify the transcription factors that are key nodes in these networks. The goal of my PhD was to study the particular gene regulatory networks that govern the decision of the V5 skin cell to divide, lose its skin fate and decide to make dopamine and glutamate sensory neurons. We chose an unbiased forward genetic screen approach coupled with whole genome sequencing of mutants derived from these screens. In the process, we found several mutants that govern this process and developed a software pipeline that simplifies the analysis of mutants for others who perform forward genetic screens.