Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation

Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation
Author :
Publisher : Universitätsverlag Göttingen
Total Pages : 148
Release :
ISBN-10 : 9783863956011
ISBN-13 : 386395601X
Rating : 4/5 (11 Downloads)

Book Synopsis Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation by : Jasper Frohn

Download or read book Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation written by Jasper Frohn and published by Universitätsverlag Göttingen. This book was released on 2023 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: To this day, the standard method for investigating biological tissue with cellular resolution is the examination under a light microscope, first denoted as histology by Karl Meyer in 1819. Despite the enormous success and importance of histology, it has two major disadvantages. Firstly, the specimen must be physically cut into thin sections due to the limited penetrating power of optical light, and secondly, additional staining of the specimen is required to achieve sufficient image contrast. Both disadvantages can be overcome by the non-destructive method of propagation-based X-ray phase-contrast tomography. While the mechanism of phase-contrast provides sufficient image contrast to image single cells, a tomographic imaging scheme with penetrating X-rays allows for an undamaged sample by virtually slicing the reconstructed 3D sample volume. In this work, the holotomography setup of the synchrotron endstation „GINIX“ (The Göttingen Instrument for Nanoscale-Imaging with X-Rays) was extended to a multi-scale X-ray phase-contrast tomography setup suitable for 3D virtual histology by adding two acquisition schemes. Compared to the existing setup, the first additional scheme is a propagation-based microtomography setup, which enlarges the reconstructed 3D volumes by a factor of approx. 64 at a fraction of the acquisition time (ca. 2 min). The second additional scheme aims for higher resolutions. To this end, the X-ray waveguide illumination was combined with photon counting detector with a large field of view and a novel phase reconstruction scheme, which is based on iterative farfield phase retrieval without an „empty-beam correction“ in the detector plane.


Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation Related Books

Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation
Language: en
Pages: 148
Authors: Jasper Frohn
Categories:
Type: BOOK - Published: 2023 - Publisher: Universitätsverlag Göttingen

DOWNLOAD EBOOK

To this day, the standard method for investigating biological tissue with cellular resolution is the examination under a light microscope, first denoted as hist
Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation
Language: en
Pages: 0
Authors: Jasper Frohn
Categories:
Type: BOOK - Published: 2023 - Publisher:

DOWNLOAD EBOOK

Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues
Language: en
Pages: 234
Authors: Marius Reichardt
Categories:
Type: BOOK - Published: 2022 - Publisher: Universitätsverlag Göttingen

DOWNLOAD EBOOK

The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. St
Nanoscale Photonic Imaging
Language: en
Pages: 634
Authors: Tim Salditt
Categories: Science
Type: BOOK - Published: 2020-06-09 - Publisher: Springer Nature

DOWNLOAD EBOOK

This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualiz
Computed Tomography
Language: en
Pages: 372
Authors: Willi A. Kalender
Categories: Medical
Type: BOOK - Published: 2011-07-07 - Publisher: John Wiley & Sons

DOWNLOAD EBOOK

The book offers a comprehensive and user-oriented description of the theoretical and technical system fundamentals of computed tomography (CT) for a wide reader